Protein_Domain

Part:BBa_K351008:Design

Designed by: Dongchan Yang   Group: iGEM10_KAIST-Korea   (2010-10-25)


Singlechain antibody for TB MPT51 protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 295
    Illegal PstI site found at 90
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 295
    Illegal PstI site found at 90
    Illegal NotI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 295
    Illegal PstI site found at 90
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 295
    Illegal PstI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is synthetic antibody. And the antigen-determine domain of antibody receptor. So if you want to change the target antibody, this sequence should be changed.


Source

It is consist of heavy and light chain of known antibody of MPT51, 16A1.

References